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Instructions

Preparing the Indole Butyric Acid start with dissolving the powder, diluting the dissolved IBA and then applying it to the plants.

Alcohol

IBA dissolves quite easily with 70% or better store bought rubbing alcohol (Isopropyl alcohol). Just combine 1 scoop of IBA powder with 0.5 teaspoon or less of alcohol and gently shake the container. In this case I used a white plastic cafeteria spoon, and half-full was about 2 ml. so very little alcohol is really needed; and the less you use the better. The powder should dissolve in a few minutes, generally less than 10. After the powder has dissolved then dilute it (instructions below).

Important: isopropyl alcohol is easy to get but if you try to mix something higher than 500 PPM some of the isopropyl alcohol & IBA will turn back into small flakes. This is because there is an upper limit on how much isopropyl alcohol & IBA mix the water can hold. Despite this limitation we like using isopropyl alcohol because most people already have it in their homes. But since the PPM will be lower you can just use the solution longer or more often.

Useful: We also used 99% methanol alcohol to dissolve the IBA powder. It worked just as well as the Isopropyl alcohol and we where able to create a 1000 PPM solution with. When using Isopropyl alcohol we could only create a 500 PPM solution.

Useful: In a later test we first dissolved on scoop. Once the first scoop was dissolved we were still able to dissolve a second scoop without using more isopropyl alcohol.

Ammonia

Ammonia can also be used to dissolve Indole Butyric Acid. Again, 1 scoop of powder was dissolved in less than half a spoon (the spoon was a standard white plastic cafeteria spoon that half full holds about 2 ml of ammonia).

After dissolving the Indole Butyric Acid (instructions are above) add the dissolved Indole Butyric Acid to water - do not add the water to the dissolved Indole Butyric Acid. To determine how much water to use first determine what plants you will use the Indole Butyric Acid for and what PPM you will need. Normally, you can divide it in the categories below. As an example, 1 scoop of powder in about 4 ounces of water is 500 PPM.

Important: In one experiment we also tried to create a 1000 PPM solution but when we added the dissolved powder to water some flakes formed. This is because there is a limit to how much dissolved IBA water can hold and if you try to add more the extra IBA turns back into powder and alcohol flakes. In this particular case we used alcohol to dissolve the IBA powder. As a follow-up we will be testing different ideas.

Important: Instead of using a high PPM of IBA you can get just a good a result if you use a lower PPM for longer. Also, instead of using a high dosage once you can also use a lower dosage twice. An example might be that instead of using 1000 PPM for 10 seconds you could also use 500 PPM for 30 seconds, or use 500 PPM twice in two days.

Recommended Concentration
Please note that generally, without using some other chemicals it is dificult to mix a solution greater than 500 PPM. It is actually better to use a lower PPM (such as 500 PPM) and to leave the plant cutting in the solution longer.
Herbaceous Cuttings	1000 PPM
Softwood	2000 to 3000 PPM
Semi-Hardwood	2000 to 4000 PPM
Hardwood	3000 to 6000 PPM

Once you decide what concentration you will need just follow the table below to make as much solution as you need.

Indole Butyric Acid Mixing Fill the scoop to the top but not above the rim.
How Much Powder To use		How Much Water To use
		100 PPM			200 PPM			300 PPM
Scoops	Grams	Liter	Ounce (US)	Gallon	Liter	Ounce (US)	Gallon	Liter	Ounce (US)	Gallon
1	0.055	0.54	18.26	0.14	0.27	9.13	0.07	0.18	6.09	0.05
2	0.110	1.08	36.51	0.28	0.54	18.26	0.14	0.36	12.17	0.09
3	0.165	1.63	55.11	0.42	0.81	27.39	0.21	0.54	18.26	0.14
4	0.220	2.17	73.37	0.56	1.08	36.51	0.28	0.72	24.34	0.19
5	0.275	2.71	91.63	0.70	1.35	45.64	0.35	0.90	30.43	0.23
6	0.330	3.25	109.88	0.85	1.63	55.11	0.42	1.08	36.51	0.28
7	0.385	3.79	128.14	0.99	1.90	64.24	0.49	1.26	42.60	0.33
8	0.440	4.33	146.40	1.13	2.17	73.37	0.56	1.44	48.69	0.37
9	0.495	4.88	164.99	1.27	2.44	82.50	0.63	1.63	55.11	0.42
10	0.550	5.42	183.25	1.41	2.71	91.63	0.70	1.81	61.20	0.47
How Much Powder To use		How Much Water To use
		400 PPM			500 PPM			750 PPM
Scoops	Grams	Liter	Ounce (US)	Gallon	Liter	Ounce (US)	Gallon	Liter	Ounce (US)	Gallon
1	0.055	0.14	4.73	0.04	0.11	3.72	0.03	0.07	2.37	0.02
2	0.110	0.27	9.13	0.07	0.22	7.44	0.06	0.14	4.73	0.04
3	0.165	0.41	13.86	0.11	0.33	11.16	0.09	0.22	7.44	0.06
4	0.220	0.54	18.26	0.14	0.43	14.54	0.11	0.29	9.80	0.08
5	0.275	0.68	22.99	0.18	0.54	18.26	0.14	0.36	12.17	0.09
6	0.330	0.81	27.39	0.21	0.65	21.98	0.17	0.43	14.54	0.11
7	0.385	0.95	32.12	0.25	0.76	25.70	0.20	0.51	17.24	0.13
8	0.440	1.08	36.51	0.28	0.87	29.41	0.23	0.58	19.61	0.15
9	0.495	1.22	41.25	0.32	0.98	33.13	0.25	0.65	21.98	0.17
10	0.550	1.35	45.64	0.35	1.08	36.51	0.28	0.72	24.34	0.19
How Much Powder To use		How Much Water To use
		1000 PPM			1500 PPM			2000 PPM
Scoops	Grams	Liter	Ounce (US)	Gallon	Liter	Ounce (US)	Gallon	Liter	Ounce (US)	Gallon
1	0.055	0.05	1.69	0.01	0.04	1.35	0.01	0.03	1.01	0.01
2	0.110	0.11	3.72	0.03	0.07	2.37	0.02	0.05	1.69	0.01
3	0.165	0.16	5.41	0.04	0.11	3.72	0.03	0.08	2.70	0.02
4	0.220	0.22	7.44	0.06	0.14	4.73	0.04	0.11	3.72	0.03
5	0.275	0.27	9.13	0.07	0.18	6.09	0.05	0.14	4.73	0.04
6	0.330	0.33	11.16	0.09	0.22	7.44	0.06	0.16	5.41	0.04
7	0.385	0.38	12.85	0.10	0.25	8.45	0.07	0.19	6.42	0.05
8	0.440	0.43	14.54	0.11	0.29	9.80	0.08	0.22	7.44	0.06
9	0.495	0.49	16.57	0.13	0.32	10.82	0.08	0.24	8.11	0.06
10	0.550	0.54	18.26	0.14	0.36	12.17	0.09	0.27	9.13	0.07

The generally accepted method of applying the dissolved and diluted Indole Butyric Acid is by soaking the cutting in the solution for a few minutes. It is always useful to try different soaking periods to find out exactly which is best for your type of plant. For example, you can try some cuttings for 5, 10, 15 and 20 minutes the first time.