Plant Cloning With Brassinolide

I've started to do some testing of root development substances and I've come up with some interesting initial research. So far I've been mostly working with brassinolide and I had great results with mint and basil while yams showed no real benefit to brassinolide. The tests were very simple. I put cuttings in water with some brassinolide in it and waited.

First the bad. I tested yam cuttings at 0.20,0.10 and 0.05 PPM. At 0.2 PPM the cutting actually died ... not a good thing. I tried another cutting at 0.10 PPM and while the cutting survived it seemed to develop roots at bit slower than the control cutting. Then I tried 2 cuttings at 0.05 PPM and they showed root development that seem a bit better than the two control plants. I need to do a lot more tests with a lot more cuttings at PPM lower than 0.05 PPM. I will be doing them once I have more plants to work with.

Next I tested some basil cuttings at 0.06, 0.03 and 0 PPM with 8 cuttings. Again, not enough but that's all I had to work with. After 11 days the 0.06 PPM was much better while the 0 PPM cuttings did much worse. I will have to run more tests with a lot more cuttings.

Finally I worked with 4 mint cuttings with 0.03 and 0 PPM. Here I found that the 0.03 PPM cuttings did much better than the two cuttings in plain water so I need to do volume testing at higher PPM.

After testing 20 different cuttings I would expect that brassinolide at 0.04 PPM is a safe and effective for helping cuttings develop roots. I currently have a lot more testing to do with a lot more cuttings.

To figure out how to mix brassinolide to get different PPM level go here.